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Southern Blot Analysis

We have performed Southern blot for our customers in different research projects:

Gene targeting ES cell positive clone screening and verification
Verification and off-targeting detection of gene editing by CRISPR/Cas9 technology
Detection of clonotypic IgH rearrangement and hypermutation
Verification of transgene integration in GMO plant
Verification of gene-targeting mouse offspring
Verificaiton of passage stability of stable cell line expressing recombinant protein
Evaluation of size of mitochondrial genomes
Detection of trinucleotide repeat expansion in trinucleotide repeat disorder
Southern blot data is the most convincing evidence for genome integrity or mutation

Comparison of gene targeting positive clone screening by PCR and Southern blot

 
PCR
Southern blot
short arm integration verification
+
+
long arm integration verification
difficult; need long range PCR
+
wild type cell contamination detection
-
+
random integration detection
-
+

Typical image of our Southern blot

#1 provider in the field with 100% customer satisfaction

“Outstanding service! Would use again and would highly recommend to others.” -Anna Mandinova from Broad Institute

“Good turnaround time and good quality of work. We will use the service again if we have Southern projects.” -Weiguo Shu from ATCC

“Excellent data!” -Jianjun Zhao from Dana-Farber Cancer Institute

To stand behind our high quality of the service, there will be no charge for your Southern project if we cannot deliver the results with our designed probes and purified genomic DNA.

Please contact us to discuss your Southern project or fill out Southern Quotation Request Form to request a quote.

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